Fig. 1.

Optimal temperature of the LAMP assay for detecting PPV.

M: DL2000 DNA marker; N: no template control; LAMP reactions were carried out at 54 ℃, 54.5 ℃, 55.2 ℃, 56.1 ℃, 57.5 ℃, 58.7 ℃, 59.6 ℃, 60.9 ℃, 62.1 ℃, 63.1 ℃, 63.7 ℃ and 64 ℃, respectively. The other 2 reactions with and without inner set of primers were carried out at 59.0 ℃ as control. Plasmids containing PPV VP1 gene fragment with the concentration 10⁴ copies per μL were as template. All reactions were incubated at above temperature for 50 min and terminated at 80 ℃ for 3 min. The amplification products were detected by 2 % (w/v) agarose gel electrophoresis staining with Goldview.