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Sensitivity of the PPV LAMP Assay.
M: DL2000 DNA marker; N: no template control; LAMP was carried out with 107 to 1 copy of plasmids of PPV as template, respectively.
All reactions were carried out at 59.0 ℃ for 50 min and terminated at 80 ℃ for 3 min.
Results on 2 % (w/v) agarose gel electrophoresis staining with Goldview.
Visual results by adding SYBR Green I.
