Fig. 5.

Comparison between samples of genomic DNA and serum for the LAMP assay.

M: DL2000 DNA marker; Lane 1, 2 and 3: LAMP reactions were carried out with 3 samples of serum, respectively; Lane 4, 5 and 6: LAMP reactions were carried out with genomic DNA of the same 3 samples, rspectively; Lane 7: positive control; Lane 8: no template control.

All reactions were carried out at 59.0 ℃ for 50 min and terminated at 80 ℃ for 3 min. The concentration of genomic DNA templates of serum samples is 10 ng per μL. Plasmids containing PPV VP1 gene fragment with the concentration 10⁴ copies per μL were as positive control.

Upper: Results on 2 % (w/v) agarose gel electrophoresis staining with Goldview.

Lower: Visual results by adding SYBR Green I.