TABLE 2.
Gene (cluster) | SG | FG-C | FG-WS | FG-WB | FG-WSWB | P |
MYH13 (1) | 2.10 ± 0.45 (a) | 0.98 ± 0.25 (b) | 0.54 ± 0.13 (bc) | 0.30 ± 0.07 (c) | 0.29 ± 0.05 (c) | 0.005 |
MYH1E (1) | 2.49 ± 0.22 (a) | 0.66 ± 0.20 (b) | 0.29 ± 0.08 (bc) | 0.17 ± 0.05 (c) | 0.21 ± 0.04 (c) | 0.0002 |
MYH1F (1) | 1.39 ± 0.24 (a) | 0.86 ± 0.15 (ab) | 0.60 ± 0.11 (b) | 0.47 ± 0.18 (b) | 0.56 ± 0.13 (b) | 0.02 |
ANKRD46 (1) | 713.6 ± 222.7 (a) | 5.72 ± 2.86 (b) | 1.86 ± 0.45 (bc) | 1.36 ± 0.61 (c) | 0.86 ± 0.16 (c) | ≤0.0001 |
MYOCD (1) | 1.87 ± 0.13 (a) | 0.77 ± 0.19 (b) | 0.53 ± 0.06 (b) | 0.34 ± 0.03 (c) | 0.55 ± 0.09 (bc) | 0.0002 |
TUBB4B (2) | 0.57 ± 0.07 (b) | 0.85 ± 0.13 (a) | 0.93 ± 0.09 (a) | 1.15 ± 0.17 (a) | 1.03 ± 0.12 (a) | 0.01 |
MYH1B (2) | 0.05 ± 0.01 (b) | 0.96 ± 0.28 (ab) | 0.75 ± 0.17 (a) | 0.57 ± 0.16 (a) | 1.26 ± 0.18 (a) | ≤0.0001 |
CTGF (4) | 0.11 ± 0.03 (d) | 0.30 ± 0.05 (c) | 0.61 ± 0.16 (bc) | 0.86 ± 0.12 (ab) | 1.46 ± 0.27 (a) | ≤0.0001 |
FN1 (4) | 0.11 ± 0.02 (d) | 0.45 ± 0.21 (c) | 0.62 ± 0.14 (bc) | 1.18 ± 0.25 (ab) | 1.74 ± 0.24 (a) | ≤0.0001 |
FASN (5) | 0.31 ± 0.03 (c) | 0.61 ± 0.12 (b) | 0.87 ± 0.14 (ab) | 0.91 ± 0.11 (ab) | 1.41 ± 0.38 (a) | 0.0007 |
ANTXR1 (5) | 1.00 ± 0.14 (b) | 0.70 ± 0.22 (b) | 0.73 ± 0.19 (b) | 0.85 ± 0.18 (b) | 2.04 ± 0.48 (a) | 0.04 |
PPARG (5) | 0.31 ± 0.03 (c) | 0.69 ± 0.23 (bc) | 0.70 ± 0.16 (b) | 0.60 ± 0.22 (bc) | 2.04 ± 0.56 (a) | 0.002 |
COL6A3 (5) | 0.39 ± 0.05 (c) | 0.71 ± 0.18 (bc) | 0.82 ± 0.12 (b) | 1.05 ± 0.13 (b) | 1.53 ± 0.15 (a) | 0.0005 |
PCNA (6) | 1.19 ± 0.13 (c) | 0.78 ± 0.12 (bc) | 0.79 ± 0.12 (b) | 1.01 ± 0.13 (b) | 1.30 ± 0.20 (a) | 0.05 |
MyoD | 0.84 ± 0.08 | 0.71 ± 0.09 | 0.84 ± 0.20 | 0.82 ± 0.11 | 0.75 ± 0.11 | NS |
MYF5 | 1.25 ± 0.19 | 1.63 ± 0.34 | 1.08 ± 0.15 | 1.53 ± 0.41 | 1.76 ± 0.38 | NS |
PITX2 | 0.67 ± 0.20 | 0.82 ± 0.17 | 1.19 ± 0.33 | 1.65 ± 0.40 | 1.06 ± 0.17 | NS |
DAG1 | 1.05 ± 0.13 | 0.89 ± 0.13 | 0.86 ± 0.09 | 0.80 ± 0.10 | 1.13 ± 0.40 | NS |
PNPLA7 | 1.14 ± 0.12 | 0.81 ± 0.10 | 0.74 ± 0.06 | 0.85 ± 0.07 | 0.91 ± 0.09 | NS |
LRSAM | 0.75 ± 0.08 | 0.78 ± 0.10 | 0.91 ± 0.10 | 0.95 ± 0.11 | 1.07 ± 0.14 | NS |
ADIPOQ | 0.84 ± 0.18 | 0.68 ± 0.10 | 0.77 ± 0.04 | 0.62 ± 0.08 | 0.95 ± 0.27 | NS |
FBN1 | 1.12 ± 0.26 | 0.55 ± 0.12 | 0.66 ± 0.08 | 0.64 ± 0.09 | 1.35 ± 0.55 | NS |
DYSF | 0.99 ± 0.09 | 0.86 ± 0.06 | 0.82 ± 0.06 | 0.76 ± 0.04 | 0.88 ± 0.10 | NS |
ANKRD1 | 0.51 ± 0.11 | 0.73 ± 0.18 | 0.68 ± 0.20 | 0.68 ± 0.17 | 1.17 ± 0.23 | NS |
Data are presented as mean ± SEM. Within a line, different letters indicate significant differences between groups (P ≤ 0.05). 1SG = slow-growing genotype, FG-C = fast-growing genotype macroscopically free of defects, FG-WS = fast-growing genotype affected by White Striping, FG-WB = fast-growing genotype affected by Wooden Breast, FG-WSWB = fast-growing genotype affected by both White Striping and Wooden Breast. 2Absolute mRNA levels of targeted genes were corrected for 18S ribosomal RNA levels to give a relative mRNA level.