Table 1.
PCR primers used for quantitative RT-PCR.
| Gene primer name1 | Primer sequence |
|---|---|
| CDH1_FP | 5’-AGGCCAAGCAGCAGTACATT |
| CDH1_RP | 5’-ATTCACATCCAGCACATCCA |
| TWIST1_FP | 5’-GCCAATCAGCCACTGAAAGG |
| TWIST1_RP | 5’-TGTTCTTATAGTTCCTCTGATTGTTACCA |
| cMYC_FP | 5’-CGACGAGACCTTCATCAAAA |
| cMYC_RP | 5’-TGCTGTCGTTGAGAGGGTAG |
| CCDN2_FP | 5’-TGATGGGGAAGGAGTACAAA |
| CCDN2_RP | 5’-TTGGAAGAAGAAGCAGGATG |
| MMP7_FP | 5’-AGCCAAACTCAAGGAGATGC |
| MMP7_RP | 5’-GCCAATCATGATGTCAGCAG |
| MMP2_FP | 5’-TTGACGGTAAGGACGGACTC |
| MMP2_RP | 5’-ACTTGCAGTACTCCCCATCG |
| Axin2_FP | 5’-CTGGCTCCAGAAGATCACAAA |
| Axin2_RP | 5’-GTGACAACCAACTCACTCGC |
| CDH2_FP | 5’-AGCCAACCTTAACTGAGGAGT |
| CDH2_RP | 5’-GGCAAGTTGATTGGAGGGATG |
| COL1A1_FP | 5’-TGGCCTCGGAGGAAACTTT |
| COL1A1_RP | 5’-TCCGGTTGATTTCTCATCATAGC |
1
FP and RP denote forward and reverse primer sequence.