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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: Mol Genet Metab. 2018 Oct 22;128(3):391–395. doi: 10.1016/j.ymgme.2018.10.005

Fig. 3.

Fig. 3.

Western Blot of MFRN1/FECH/GAPDH in patients with protoporphyria due to mutations in either FECH (non-sense or C411G) or gain-of-function mutations in ALAS2. Total protein, 80 μg, from EBVTL of patients were subjected to SDS-PAGE then transferred to PVDF membranes. Blots were developed with anti-FECH (1:1000), anti-MFRN1 (1:1000), anti-GAPDH (1:2000) antibodies followed by a secondary horseradish peroxidase (HRP)-labelled goat anti-rabbit secondary antibody (1:3000).