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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: J Vis Exp. 2019 Jun 7;(148):10.3791/59526. doi: 10.3791/59526

Figure 1: Schematic of a 2pFLIM system.

Figure 1:

2pFLIM can be implemented on a conventional two-photon microscope by the addition of the yellow highlighted hardware components: a photon timing counting module, a low-noise fast photomultiplier tube (PMT), a photodiode (only needed if the laser does not have an output signaling for laser timing), and an optional signal splitter. This figure has been modified from Ma et al.17.