Skip to main content
. 2020 Jul 1;15(7):e0235573. doi: 10.1371/journal.pone.0235573

Fig 2. Increased cell viability and sphere formation in Kras-mutant pancreatic ductal cells in a hyperglycemic environment.

Fig 2

The pancreatic ductal cancer cell lines PANC-1, mPKC1, and BxPC3 were maintained in a low-glucose (5.5 mM) or high-glucose (25 mM) DMEM for 28 days prior to the experiment. (A) Time course of the viability of pancreatic ductal cells grown in the low- or high-glucose medium, measured by the WST assay (n = 8 each). (B) Quantification of the sphere-forming capacity of pancreatic ductal cells 7 days after seeding (n = 6 each); Scale bar, 50 μm. (C) Murine-derived mPKC1 cells were orthotopically injected into the tail of the mouse pancreas. Tumor weight was measured 14 days after injection (n = 10 each). (D) BrDU cell proliferation assay of pancreatic ductal cells in the low- or high-glucose medium 48 hr after seeding (n = 8 each). (E) Annexin V assay of pancreatic ductal cells and the percentage of annexin V-positive/PI-negative cells (n = 3 each). Error bars: mean+s.d. *P<0.05.