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. 2020 Jul 1;15(7):e0235573. doi: 10.1371/journal.pone.0235573

Fig 3. Hyperglycemia elevates MYC expression in combination with STAT3 activation.

Fig 3

All pancreatic ductal cell lines (PANC-1, mPKC1, and BxPC3) were preconditioned with a low-glucose (5.5 mM) or high-glucose (25 mM) DMEM for 28 days. (A) Western blot analysis of the levels of pSTAT3, STAT3, MYC, pERK, ERK, pAKT, AKT, Zeb1, and beta-actin in the low- or high-glucose medium-maintained cells. Quantification of pSTAT3/STAT3, MYC/beta-actin, and pERK/ERK (n = 3 each); error bars: mean+s.d. (B) Immunostaining of control KP vs. STZ KP pancreata for pSTAT3, MYC, and pERK and the associated scoring. The pancreata from the STZ KP and vehicle KP mice were sectioned in advance. Scale bar, 200 μm; n = 8 each; *P<0.05.