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. 2020 Jul 1;15(7):e0235573. doi: 10.1371/journal.pone.0235573

Fig 4. STAT3 inhibition and its relationship with MYC in pancreatic ductal cells.

Fig 4

Kras-mutant PANC-1 and mPKC1 cells were incubated with a low-glucose (5.5 mM) or high-glucose (25 mM) DMEM for 28 days. The cells were then treated with 1 μM STAT3 inhibitor STATTIC. (A) Western blot analysis of PANC-1 and mPKC1 cells with or without 1 μM STATTIC treatment. The levels of pSTAT3, STAT3, MYC, pERK, ERK, and beta-actin are shown. Quantification of pSTAT3/STAT3, MYC/beta-actin, and pERK/ERK (n = 3 each); error bars: mean+s.d. (B) Time course of the viability of PANC-1 and mPKC1 cells incubated with or without 1 μM STATTIC, measured by the WST assay (n = 8 each); error bars: mean+s.d. (C) Quantification of the sphere-forming capacity of PANC-1 and mPKC1 cells with or without 1 μM STATTIC treatment (n = 6 each); Scale bar, 50 μm; Tukey’s HSD test; *P<0.05.