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A total of 256 combinations of selective EcoRI+2 and MseI+2 primer pairs were employed to generate 2,304 virtual AFLP profiles AFLPinSilico (A) and ISIF (B). The dots located on the left X-axis represent the number of fragments generated for each combination. The bold bar represents the average of fragments generated for all combinations. The white dots located on the right X-axis represent the genome size, estimated by whole genome sequencing.
