Table 3. Summary of polymorphism statistics calculated for different pairs of selective primers (EcoRI+2 and MseI+2) of Sporothrix species.
#1 EcoRI-GA/MseI-AA | |||||||||
Species | Scored bands | H | PIC | E | Havp | MI | D | Rp | Error % |
S. brasiliensis | 62 | 0.4991 | 0.3745 | 29.6667 | 0.0009 | 0.0265 | 0.7715 | 21.3333 | 1.12 |
S. schenckii | 84 | 0.4995 | 0.3747 | 40.6250 | 0.0007 | 0.0302 | 0.7665 | 38.2500 | 1.23 |
S. globosa | 43 | 0.3918 | 0.3151 | 31.5000 | 0.0015 | 0.0478 | 0.4641 | 9.6667 | 1.05 |
S. mexicana | 45 | 0.1435 | 0.1332 | 41.5000 | 0.0016 | 0.0661 | 0.1503 | 7.0000 | 0.00 |
Overall | 135 | 0.3857 | 0.3113 | 35.2222 | 0.0001 | 0.0037 | 0.9320 | 63.1852 | 1.04 |
#2 EcoRI-AA/MseI-AA | |||||||||
Species | Scored bands | H | PIC | E | Havp | MI | D | Rp | Error % |
S. brasiliensis | 56 | 0.4997 | 0.3749 | 28.6667 | 0.0010 | 0.0284 | 0.7385 | 16.8889 | 0.00 |
S. schenckii | 63 | 0.4854 | 0.3676 | 26.1250 | 0.0010 | 0.0252 | 0.8285 | 26.7500 | 0.47 |
S. globosa | 34 | 0.4192 | 0.3313 | 23.8333 | 0.0021 | 0.0490 | 0.5097 | 10.3333 | 0.64 |
S. mexicana | 37 | 0.0267 | 0.0263 | 36.5000 | 0.0004 | 0.0132 | 0.0270 | 1.0000 | 0.00 |
Overall | 137 | 0.3287 | 0.2747 | 28.4074 | 0.0001 | 0.0025 | 0.9570 | 52.0741 | 0.28 |
#3 EcoRI-GA/MseI-TT | |||||||||
Species | Scored bands | H | PIC | E | Havp | MI | D | Rp | Error % |
S. brasiliensis | 52 | 0.4974 | 0.3737 | 27.8889 | 0.0011 | 0.0296 | 0.7129 | 19.7778 | 0.00 |
S. schenckii | 52 | 0.4833 | 0.3665 | 21.2500 | 0.0012 | 0.0247 | 0.8336 | 17.5000 | 0.58 |
S. globosa | 28 | 0.3501 | 0.2888 | 21.6667 | 0.0021 | 0.0451 | 0.4023 | 4.0000 | 0.00 |
S. mexicana | 21 | 0.0465 | 0.0454 | 20.5000 | 0.0011 | 0.0227 | 0.0476 | 1.0000 | 0.00 |
Overall | 106 | 0.3449 | 0.2854 | 23.4815 | 0.0001 | 0.0028 | 0.9510 | 40.8889 | 0.16 |
#4 EcoRI-AA/MseI-TT | |||||||||
Species | Scored bands | H | PIC | E | Havp | MI | D | Rp | Error % |
S. brasiliensis | 44 | 0.4999 | 0.3750 | 21.7778 | 0.0013 | 0.0275 | 0.7557 | 16.4444 | 0.51 |
S. schenckii | 52 | 0.4967 | 0.3733 | 23.8750 | 0.0012 | 0.0285 | 0.7898 | 19.7500 | 0.52 |
S. globosa | 38 | 0.4038 | 0.3223 | 27.3333 | 0.0018 | 0.0484 | 0.4835 | 8.6667 | 0.00 |
S. mexicana | 28 | 0.0000 | 0.0000 | 28.0000 | 0.0000 | 0.0000 | 0.0000 | 0.0000 | 0.00 |
Overall | 111 | 0.3483 | 0.2876 | 24.9259 | 0.0001 | 0.0029 | 0.9496 | 45.6296 | 0.32 |
#5 EcoRI-GA/MseI-AG | |||||||||
Species | Scored bands | H | PIC | E | Havp | MI | D | Rp | Error % |
S. brasiliensis | 55 | 0.4993 | 0.3746 | 28.5556 | 0.0010 | 0.0288 | 0.7309 | 18.6667 | 0.78 |
S. schenckii | 52 | 0.4958 | 0.3729 | 28.3750 | 0.0012 | 0.0338 | 0.7028 | 14.2500 | 0.00 |
S. globosa | 29 | 0.4096 | 0.3257 | 20.6667 | 0.0024 | 0.0486 | 0.4933 | 10.0000 | 0.00 |
S. mexicana | 28 | 0.0000 | 0.0000 | 28.0000 | 0.0000 | 0.0000 | 0.0000 | 0.0000 | 0.00 |
Overall | 99 | 0.3908 | 0.3145 | 26.3704 | 0.0001 | 0.0039 | 0.9291 | 40.9630 | 0.29 |
#6 EcoRI-TA/MseI-AA | |||||||||
Species | Scored bands | H | PIC | E | Havp | MI | D | Rp | Error % |
S. brasiliensis | 49 | 0.4787 | 0.3641 | 19.4444 | 0.0011 | 0.0211 | 0.8431 | 21.7778 | 1.14 |
S. schenckii | 53 | 0.4639 | 0.3563 | 19.3750 | 0.0011 | 0.0212 | 0.8669 | 22.2500 | 0.00 |
S. globosa | 36 | 0.4928 | 0.3714 | 15.8333 | 0.0023 | 0.0361 | 0.8077 | 18.3333 | 0.00 |
S. mexicana | 15 | 0.0644 | 0.0624 | 14.5000 | 0.0021 | 0.0311 | 0.0667 | 1.0000 | 0.00 |
Overall | 97 | 0.3003 | 0.2552 | 17.8519 | 0.0001 | 0.0020 | 0.9662 | 33.6296 | 0.44 |
D = discriminating power; E = effective multiplex ratio; H = expected heterozygosity; Havp = mean heterozygosity; MI = marker index; PIC = polymorphism information content; Rp = resolving power.