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. 2020 Jul 1;15(7):e0235546. doi: 10.1371/journal.pone.0235546

Fig 1. Purity of the generated hResistin protein and anti-hResistin antibody.

Fig 1

(A) Coomassie-stained gel shows SDS-PAGE analysis of purified rhResistin protein. (B) Western blotting shows that the purified hResistin protein dose-dependently induced phosphorylation of Akt in the 3T3-L1 fibroblast cell line. (C) Purity of the anti-hResistin antibody and the control human IgG1 were analyzed by SDS-PAGE. (D) Size exclusion high-performance liquid chromatogram for the anti-hResistin antibody. Ab, antibody; Con, control; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; hResistin, human resistin; p-Akt, phosphorylated Akt.