Figure 4. In the presence of cognate T cells, adoptively transferred, tolerant, alloreactive B cells express early activation markers but have reduced GC differentiation.

Spleens and inguinal, axillary, and branchial lymph nodes (LNs) were harvested from MD4 hosts that received 2 × 10⁷/mouse naive B (N-B) cells or tolerant B (Tol-B) cells followed by immunization with 2 × 10⁷ B/c DSCs and analyzed on day 14 after AdTr. (A) Percentage GCs of anti-K^d (αK^d) B cells. (B) Total number of GCs of αK^d B cells/mouse (mse). (C) Percentage GCs of αI-E^d B cells. (D) Total number of GCs of αI-E^d B cells/mouse. n = 5–8/group. (E–H) Representative histograms and percentage of (E and F) Ki-67⁺ anti-tetramer (αTet) B cells and (G and H) AID⁺ αTet B cells. n = 6–7/group. (I–N) Representative histograms and MFI of (I and J) CD69, (K and L) Glut-1, and (M and N) mitochondrial mass (MM) of αTet B cells from the spleens and LNs harvested from MD4 mice that received N-B and Tol-B cells on day 3 after AdTr, and then immunized with 2 × 10⁷ B/c DSCs plus CpG. n = 5–6/group. Data were pooled from 2 independent experiments. Data are presented as the mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001 by 1-way ANOVA with Bonferroni’s post hoc test.