KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| anti-Rh6 (mouse) | Stephen Britt | |
| anti-DsRed antibody (rabbit) | Clontech Laboratories, Inc. | Cat # 632496, RRID: AB_10013483 |
| Goat anti-mouse, Alexa488 | Thermo Fisher Scientific | Cat # A11001, RRID: AB_2534069 |
| Goat anti-rabbit, Alexa568 | Thermo Fisher Scientific | Cat # A11036, RRID: AB_10563566 |
| Chemicals | ||
| Sucrose | Sigma-Aldrich | Cat # S0389 |
| Aristolochic acid | Sigma-Aldrich | Cat # A9451 |
| Piperonyl acetate | Sigma-Aldrich | Cat # W291218 |
| Dry yeast | Genesee Scientific | Cat # 62–103 |
| D-(+)-glucose | Sigma-Aldrich | Cat # G8270 |
| Rice powder | United Foodstuff Company | N/A |
| Agar | BD Diagnostics | Cat # 214010 |
| Cholesterol | Sigma-Aldrich | Cat # C8667 |
| Methyl 4-hydroxybenzoate | Sigma-Aldrich | Cat # H5501 |
| Propionic acid | Sigma-Aldrich | Cat # 81910 |
| Myristic acid | Sigma-Aldrich | M3128 |
| Paraformaldehyde | Electron Microscopy Sciences | |
| Triton X-100 | Sigma | Cat # X100 |
| VECTASHIELD anti-fade mounting media | Vector Labs | Cat # H-1200 |
| Experimental Models: Organisms/Strains | ||
| Drosophila: w1118 | Bloomington Drosophila Stock Center | Cat # BL5905 |
| Drosophila: Gr66a-Gal4 (chromosome 2 insertion) | [47] | NA |
| Drosophila: Gr66a-Gal4 (chromosome 3 insertion) | Bloomington Drosophila Stock Center | Cat # BL57670 |
| Drosophila: Gr64f-Gal4 | Bloomington Drosophila Stock Center | Cat # BL57669 |
| Drosophila: UAS-TNT-E | [14] | NA |
| Drosophila: R41E1-Gal4 | Bloomington Drosophila Stock Center | Cat # BL50131 |
| Drosophila: ppk28-Gal4 | [48] | NA |
| Drosophila: TMC-Gal4 | [2] | Cat # BL62176 |
| Drosophila: UAS-tdTomato | Bloomington Drosophila Stock Center | Cat # BL36327 |
| Drosophila: UAS-tdTomato | Bloomington Drosophila Stock Center | Cat # BL36328 |
| Drosophila: UAS-rh6 | Laboratory of Craig Montell | NA |
| Drosophila: UAS-GCaMP6f | Bloomington Drosophila Stock Center | Cat # BL42747 |
| Drosophila: nompC-lexA | [50] | NA |
| Drosophila: LexAop-rCD2::RFP, UAS-mCD8::GFP | Bloomington Drosophila Stock Center | Cat # BL67093 |
| Drosophila: ninaEI17 | Bloomington Drosophila Stock Center | Cat # BL5701 |
| Drosophila: ninaD1 | Bloomington Drosophila Stock Center | Cat # BL42244 |
| Drosophila: rh21 | Laboratory of Craig Montell | N/A |
| Drosophila: rh32 | This paper | N/A |
| Drosophila: rh41 | [49] | N/A |
| Drosophila: Gr5a-GAL4 | Bloomington Drosophila Stock Center | Cat # BL57592 |
| Drosophila: rh5LexA | This paper | N/A |
| Drosophila: rh61 | Drosophila Genomics and Genetic Resources (Kyoto Stock Center) | Cat # 109600 |
| Drosophila: rh6G | Bloomington Drosophila Stock Center | Cat # BL66672 |
| Drosophila: rh71 | [51] | N/A |
| Drosophila: rh22 | Laboratory of Craig Montell | N/A |
| Drosophila: trpMB03672 | [52] | N/A |
| Drosophila: trplMB10533 | Bloomington Drosophila Stock Center | Cat # BL29134 |
| Drosophila: trpA11 | Bloomington Drosophila Stock Center | Cat # BL26504 |
| Drosophila: Ir21a123 | [23] | N/A |
| Drosophila: Ir21aΔ1 | [23] | N/A |
| Drosophila: Ir25a2 | Bloomington Drosophila Stock Center | Cat # BL41737 |
| Drosophila: Ir93aMI05555 | Bloomington Drosophila Stock Center | Cat # BL42090 |
| Drosophila: tub-Gal80ts | Bloomington Drosophila Stock Center | Cat # BL 7018 |
| Oligonucleotides | ||
| rh32 genotyping PCR primer sequences P1: TACTGCAACCCAAAATGGTCA | This paper | N/A |
| rh32 genotyping PCR primer sequences P2: TCCACGTTCATCTTCTTGGCC | This paper | N/A |
| rh5LexA genotyping PCR primer sequences P1: GGCACCTATCTACTATCACGCCG | This paper | N/A |
| rh5LexA genotyping PCR primer sequences P2: ACGTCCGTCTATTGGATTGG | This paper | N/A |
| rh5LexA genotyping PCR primer sequences P3: GAACCTGGTACATCAAATACCCTTGG | This paper | N/A |
| rh5LexA genotyping PCR primer sequences P4: ATTCGCAACTGGGTCTCAAG | This paper | N/A |
| rh5LexA genotyping PCR primer sequences P5: TCCATGTCCCATTGGAAAGAC | This paper | N/A |
| rh5LexA genotyping PCR primer sequences P6: TATTTTCTGGCCGCCTAATG | This paper | N/A |
| Recombinant DNA | ||
| pBPLexA::p65Uw | Donor vector | Addgene Plasmid # 26231 |
| pU6-BbsI-chiRNA | gRNA vector | Addgene Plasmid # 45946 |
| Other | ||
| Glass capillaries | World Precision Instruments | Cat # 1B150F-3 |