mGluR1 is required for the E2-induced enhancement of ethanol excitation. A, Rate meter graph represents the response to 40–120 mm ethanol of a representative neuron from an OVX mouse treated with vehicle (VEH) before and after treatment with the mGluR1 antagonist JNJ 16259685. B, Ethanol concentration-response graph of neurons (n = 5) from OVX mice treated with VEH showing responses before (DMSO vehicle) and after slice treatment with JNJ 16259685. C, Rate meter graph represents the response to 40-120 mm ethanol of a representative neuron from an E2-treated OVX mouse before and after treatment with JNJ 16259685. D, Ethanol concentration-response graph of neurons (n = 6) from EII-treated OVX mice before and after treatment of slices with JNJ 16259685. The ethanol excitation of neurons from E2-treated OVX mice was significantly reduced after JNJ 16259685. *p < 0.05, main effect of treatment (two-way repeated-measures ANOVA).