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. 2020 Jul 1;20:306. doi: 10.1186/s12870-020-02512-2

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 9 — Effects of GA, PAC and G + P on the expression of genes involved in GA biosynthesis. Fold changes of early steps genes (a-d), CoGA20ox7 (e), CoGA3ox2 (f), CoGA2 oxidases (g-h). The expression was analyzed by using quantitative RT-PCR method and relative expression value was determined by 2^-ΔΔCT method. Normalization was done against GAPDH gene. Expression level of tissue treated with water considered as control. The CT value of each gene was the average of three biological and three technical replicates. Columns at each observation followed by the different letter are significantly different according to LSD test at P ≤ 0.05. Vertical bars indicate standard errors