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. 2020 Jul 1;21:157. doi: 10.1186/s13059-020-02058-4

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Culture system for modelling EHT in vitro. a Schematic of the differentiation protocol used to model EHT with hPSCs. D, day. b Representative images of cells progressing through EHT. Scale bar is 400 μm. c Quantification of colonies generated in a CFU assay using cells collected at EHT D5. Data represent means ± SEM of 3 independent experiments. BFU-E, burst forming unit-erythroid; CFU-E, colony forming unit-erythroid; CFU-GM, colony forming unit-granulocyte-macrophage; CFU-G, colony forming unit-granulocyte; CFU-M, colony forming unit-macrophage; CFU-GEMM, colony forming unit-granulocyte-erythrocyte-macrophage-megakaryocyte. d, e Flow cytometry analysis of d cells at D8/EHT D0 and e during EHT culture at multiple time points. Results are representative of at least 3 independent experiments