Table 2.
Primers used for qPCR
| Gene | Forward primer (5′–3′) | Reverse primer (5′–3′) |
|---|---|---|
| CDH5 | TGGCCAGCTGGTCCTGCAGAT | TGCCCGTGCGACTTGGCATC |
| CDK1 | GGAAGGGGTTCCTAGTACTGC | AAGCACATCCTGAAGACTGACT |
| CDK2 | CTCCAGGGCCTAGCTTTCTG | CCGGCGAGTCACCTCATGG |
| CDK4 | TGAGGGGGCCTCTCTAGCTT | CAAGGGAGACCCTCACGCC |
| CDK6 | ACAGAGCACCCGAAGTCTTG | GGGAGTCCAATCACGTCCAA |
| CDKN1A/p21 | GGCAGACCAGCATGACAGAT | GATGTAGAGCGGGCCTTTGA |
| CDKN1B/p27 | TAATTGGGGCTCCGGCTAAC | GAAGAATCGTCGGTTGCAGGT |
| CDKN1c/p57 | GCTGCGGTGAGCCAATTTAG | AACAAAACCGAACGCTGCTC |
| CYCLIN B1 | CGCCTGAGCCTATTTTGGTTG | AGTGACTTCCCGACCCAGTA |
| CYCLIN D1 | GCTGTGCATCTACACCGACA | AAATGAACTTCACATCTGTGGCA |
| CYCLIN D2 | GCCACCGACTTTAAGTTTGC | CGGTACTGCTGCAGGCTATT |
| CYCLIN D3 | TGTGCTACAGATTATACCTTTGCC | GCTTCGATCTGCTCCTGACA |
| CYCLIN E1 | GACGGGGAGCTCAAAACTGA | TCGGGCTTTGTCCAGCAAAT |
| GATA1 | CTACACCAGGTGAACCGGC | CTTTTCCAGATGCCTTGCGG |
| GATA2 | CTGTTCAGAAGGCCGGGAG | AATTTGCACAACAGGTGCCG |
| GFI1 | ATCCACACTGGTGAGAAGCC | GCTGCCCTCTGTAGTGTTGT |
| LMO2 | CAGAACATTGGGGACCGCTA | GTCTTGCCCAAAAAGCCTGA |
| KLF1 | CCGAGGAAGAGGAGGCTTGAG | GGAAGTCATCCTGTGTGTCCG |
| MEIS1 | GCGCAAAGGTACGACGATCT | GGTACTGATGCGAGTGCAGA |
| MYB | GCTACTGCCTGGACGAACTG | GTTGTTAACAGTGGGCTGGC |
| PECAM1 | CAGGCGCCGGGAGAAGTGAC | CGTCCAGTCCGGCAGGCTCT |
| RB1 | CTGTGGATGGAGTATTGGGAGG | TCTCATCTAGGTCAACTGCTGC |
| RBL1/p107 | AGCAGAGGAGGATTCCTTGCAG | GGGCACATAATCGCATTGGC |
| RBL2/p130 | GCTACACGCTGGAGGGAAAT | TCCTTCCACTGTCCCTTTGC |
| RPLP0 | GGCGTCCTCGTGGAAGTGAC | GCCTTGCGCATCATGGTGTT |
| RUNX1 | CATCGCTTTCAAGGTGGTGG | CATGGCTGCGGTAGCATTTC |
| SPI1 | CCCCACGACCGTCCAG | GTAATGGTCGCTATGGCTCTCC |
| TAL1 | TACTGATGGTCCCCACACCA | CCAGGCGGAGGATCTCATTC |
| VWF | TTACGTGGGTGGGAACATGG | TCTGTGGTGACTGTGCCATC |