Fig. 4.

Fyn Kinase Inhibitor Does Not Alter Tau pY18 but Does Reduce Fyn/Tau Colocalization. (a) Representative images of immunofluorescent staining for pY18 in the dentate gyrus (DG) of the hippocampus in 9-month-old PS19 mice after 7 months of AZD0530 or Vehicle treatment. Scale bar, 20 μm. (b) Quantification of pY18-positive area (%) in the dentate gyrus (DG) of the hippocampus in 9-month-old PS19 and WT mice after 7 months of treatment. Data are mean ± SEM. n = 7–10, each dot is the average of three sections from one mouse. One-way ANOVA with Sidak’s multiple comparisons test. (c) Representative images of immunofluorescent staining for pY18 in the CA1 of the hippocampus in 9-month-old PS19 mice after 7 months of treatment. Scale bar, 20 μm. (d) Quantification of pY18-positive (%) area in the CA1 of the hippocampus in 9-month-old PS19 and WT mice after 7 months of treatment. Data are mean ± SEM. n = 7–10, each dot is the average of three sections from one mouse. Each point is the average of 3 slices from the same animal. One-way ANOVA with Sidak’s multiple comparisons test. (e) Representative immunofluorescent images of PLA from HEK-293 T cells expressing human Fyn and Tau treated with 2 μM AZD0530 in DMSO or DMSO (control) for 24 h. Fyn-Tau PLA in green are sites of Fyn and Tau interaction. Tau-positive area are in red and Fyn-positive area are in magenta. Scale bar, 20 μm. (f) Quantification of the area of Fyn- Tau PLA density (%) within Tau-positive area of HEK-293 T cells expressing human Fyn and Tau, normalized to the condition with no treatment. Data are mean ± SEM. n = 3. Each point represents the average of four images taken per experimental condition. *p < 0.05; Unpaired t-test. (g) Quantification of the mean intensity of Tau (%) within Tau-positive area of HEK-293 T cells, normalized to the condition with no treatment. Data are mean ± SEM. n = 3. Each point represents the average of four images taken per experimental condition. Unpaired t-test. (h) Quantification of mean intensity of Fyn (%) within Tau-positive area of HEK-293 T cells, normalized to the condition with no treatment. Data are mean ± SEM. n = 3. Each point represents the average of four images taken per experimental condition. Unpaired t-test. (i) Quantification of the percent Fyn-Tau PLA density of HEK-293 T cells expressing only human tan in Tau-positive cells, normalized to the percent density in Tau- and Fyn- transfected HEK-293 T cells. Data are mean ± SEM. n = 3. Each point represents the average of four images taken per experimental condition