Fig. 3.

Live-cell PALM microscopy with KEC. HeLa Kyoto cells transiently expressed either vimentin-Dendra2 (a) or vimentin–E3I along with K4V–Dendra2 (E3I/K4V). b Images were reconstructed from frames 1–1000 using the Haar wavelet kernel (HAWK) algorithm designed for a high-density labeling regime. The bottom row shows magnified regions from (a) and (b), marked with yellow squares. Each image was rendered from 1000 frames (~ 24 s). Images were acquired at 42 fps with 16 ms exposure time under excitation of 56 mW of 561 nm laser line. Scale bars are 10 μm