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. Author manuscript; available in PMC: 2020 Nov 18.
Published in final edited form as: Nat Neurosci. 2020 May 18;23(7):854–868. doi: 10.1038/s41593-020-0632-8

Figure 5 |. Activation of CeAGA neurons strongly reduced nociception-related behaviors in chronic constriction injury model and drove CPP.

Figure 5 |

a, Schematic of the site of chronic constriction injury of the Infraorbital Nerve.

b, Quantification of activating CeAGA induced changes in withdrawal frequency to 8 different von Frey filaments in the injured and non-injured side of the whisker pad after IoN-CCI. (Injured side, control, n=8 animals (0 ± 0 (.008g), −0.13 ± 0.13 (.02g), −0.13 ± 0.23 (.04g), −0.63 ± 0.38 (.07g), −0.50 ± 0.19 (.16g), 0.13 ± 0.30 (.40g), −0.13 ± 0.23 (.60g), 0.13 ± 0.13 (1.0g)); ChR2, n=7 animals (0 ± 0 (.008g), −1.14 ± 0.46 (.02g), −1.29 ± 0.52 (.04g), −2.00 ± 0.62 (.07g), −3.71 ± 0.71 (.16g), −5.57 ± 0.84 (.40g), −4.57 ± 0.65 (.60g), −4.00 ± 0.69 (1.0g)); two-way ANOVA; ****P<0.0001; F7,104=10.74; Un-Injured side, control, n=8 animals (0 ± 0 (.008g), −0.25 ± 0.16 (.02g), 0 ± 0.19 (.04g), −0.13 ± 0.35 (.07g), −0.88 ± 0.35 (.16g), 0 ± 0.27 (.40g), −0.13 ± 0.13 (.60g), −0.13 ± 0.13 (1.0g)); ChR2, n=7 animals (0 ± 0 (.008g), −1.29 ± 0.64 (.02g), −1.86 ± 0.67 (.04g), −2.57 ± 0.65 (.07g), −2.43 ± 0.78 (.16g), −3.14 ± 1.14 (.40g), −2.86 ± 0.94 (.60g), −0.57 ± 0.37 (1.0g)); two-way ANOVA; **P<0.01 (0.07g), ***P<0.001 (0.40g), **P<0.01 (0.60g) ; F7,104=2.393). Data are mean ± s.e.m..

c, Example heat map of CPP/CPA experiment. Control and ChR2 mice received stimulation on non-preferred side.

d, Quantification of the percent (%) of time spent on the stimulated side after chronic constriction injury (control, n=7 animals (35.92 ± 4.38 (pre), 40.17 ± 6.09 (post), ChR2, n=12 animals (37.87 ± 2.76 (pre), 52.74 ± 4.48 (post); two-way ANOVA; **P<0.01, ns, P=0.80; F1,17=7.185). Data are mean ± s.e.m..

e, Quantification of percent (%) of time eliciting spontaneous wiping after chronic constriction injury before, during and after light stimulation (control, n=7, ChR2, n=7 animals; 7 minutes baseline no light (5.75 ± 1.07 (control), 3.79 ± 0.22 (ChR2), followed by 5 minutes of light-stimulation (5.61 ± 1.20 (control), 1.33 ± 0.42 (ChR2),, and another 7 minutes post-stimulation (5.37 ± 1.16 (control), 1.73 ± 0.46 (ChR2),; two-way repeated measures ANOVA; **P<0.01, *P<0.05; F1,12=11.98). Data are mean ± s.e.m..