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. 2020 Apr 23;56(4):448–460. doi: 10.1007/s11262-020-01755-3

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Complete genome analysis of PUUV isolates. Complete PUUV genomes isolated from lung tissues and positive cell line passages were determined by HTS and dideoxy-chain termination sequencing in combination with RNA ligation to obtain complete NCRs. Nucleotide sequence insertion in the S segment NCR and amino acid exchanges in the L segment encoded RdRP are compared. Black dots indicate sequences derived from lung tissue and triangles indicate sequences obtained from cell culture passages. The complete genome of PUUV strain Astrup was used as a reference sequence ([21]; GenBank accession numbers: KJ994776-78). Coding regions of the three segments are indicated by numbers. NCR, non-coding region; M43, PUUV Osnabrück/MGN-2-R 43; V29, PUUV Osnabrück/VeroE6 29