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. 2020 Apr 23;56(4):448–460. doi: 10.1007/s11262-020-01755-3

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Hantavirus phylogenetic trees. Hantavirus phylogenetic tree of concatenated S, M, and L coding sequences of 18 published and four novel complete genomes (a). Partial S segment coding sequences of 365 nucleotides length, reconstructed with four novel and 202 published partial sequences (b). New PUUV isolates (GenBank accession numbers: MN639737-MN639742) are indicated with triangles and sequences derived from their original lung tissue (GenBank accession numbers: MN639743-MN639748) are labeled with black dots. Published sequences of other hantaviruses are labeled with GenBank accession numbers. Novel sequences are highlighted in gray. Posterior probabilities for major nodes of the maximum clade credibility phylogenetic tree are displayed. Analysis was performed by Bayesian algorithms via MrBayes v.3.2.6 (https://sourceforge.net/projects/mrbayes/files/mrbayes/) on the CIPRES online portal [28]. A mixed nucleotide substitution matrix was specified in 4 independent runs of 10⁷ generations. Scale bar indicates nucleotide substitutions per site. For clarity, previously characterized PUUV clades from other parts of Europe are shown in simplified form. CE, Central European; LAT, Latvian; ALAD, Alpe-Adrian; S-SCA, South Scandinavian; N-SCA, North Scandinavian; RUS, Russian; FIN, Finnish; DEN, Danish