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. 2020 Apr 23;56(4):448–460. doi: 10.1007/s11262-020-01755-3

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Reactivity of novel PUUV GPC-specific monoclonal antibodies with hantavirus-infected VeroE6 cells in immunofluorescence assay (IFA). Antibodies were generated by immunization of BALB/c mice with GPC-derived virus-like particles of PUUV strain Astrup. After screening and subcloning, monoclonal antibodies were tested in IFA. VeroE6 cells were infected with PUUV Osnabrück V29 isolate on coverslips and fixed for IFA after 10 days. The monoclonal antibodies were administered for 1 h at RT. Detection of the specific antibody binding was done using an anti-mouse Alexa fluor 488 conjugated antibody. After staining, coverslips were mounted on glass slides for imaging