Table 1.
Antibodies and lectins used for Western blot experiments
| Primary antibodies or lectins | Secondary antibodies or lectins | Specificity | Negative control | Positive control |
|---|---|---|---|---|
| Chicken anti-Neu5Gc IgY (1:2,000) | Peroxidase-conjugated donkey anti-chicken IgY (1:50,000) | Neu5Gc | C-P55 | CP-NeuGc |
| Human anti-αGal IgG+IgM (1.68 mg/ml) |
Peroxidase-conjugated goat anti-human IgG (1:20,000) Peroxidase-conjugated goat anti-human IgM (1:10,000) |
αGal | none | C-PGC2 |
| Anti-LDN IgM SMLDN1.1 (1:50) | Peroxidase-conjugated goat anti-mouse IgM (1:20,000) | LacdiNAc | C-P55 | CP-LDN |
| KM694 (5 μg/ml) | Peroxidase-conjugated goat anti-mouse IgM (1:20,000) | Sda | C-P55 | none |
| Anti-Lewis a (1:500) | peroxidase-conjugated goat anti-mouse IgG Fab (1:10,000) | Lewis a | C-P55 | Lea-BSA |
| Anti-Lewis b (1:1,000) | Peroxidase-conjugated goat anti-mouse IgM (1:10,000) | Lewis b | C-P55 | Leb-BSA |
| Anti-Lewis x (1:800) | Peroxidase-conjugated goat anti-mouse IgM (1:10,000) | Lewis x | C-P55 | CP-Lex |
| Mouse anti-human CD15s (1:1,000) | Peroxidase-conjugated goat anti-mouse IgM (1:10,000) | sialyl-Lewis x | C-P55 | sLex-BSA |
| Blood group Lewis y F3 (1:500) | Peroxidase-conjugated goat anti-mouse IgM (1:10,000) | Lewis y | C-P55 | CP-Lewis Y |
| MAL-1 (1 μg/ml) | Peroxidase-conjugated avidin D (1 μg/ml) | (α2,3-Neu5Ac/Gc)Galβ4GlcNAc | none | CP-C3 |
| MAL-2 (1 μg/ml) | Peroxidase-conjugated avidin D (1 μg/ml) | α2,3-sialic acids | none | CP-ext C1 |
| SNA (1 μg/ml) | Peroxidase-conjugated avidin D (1 μg/ml) | α2,6-sialic acids | none | CP-ext C1-ST6 |