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. 2020 May 29;412(19):4619–4628. doi: 10.1007/s00216-020-02711-8

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© The Author(s) 2020

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Fig. 3 — Summary of spectral pre-processing steps demonstrated by spectra of REV samples. a Raw spectra after ATR correction. b Spectra after baseline correction and normalization. c Spectra after buffer subtraction. d Zoomed spectra for the calculation of area under the curve (AUC) values of the amide I band by integration in 1700–1600 cm⁻¹ wavenumber region