Figure 3.

Nurr1 repressed the transcriptional activity of the RasGRP1 gene. (A) Schematic representation of the mouse RasGRP1 gene, indicating the position of NBRE in the RasGRP1 intron. The second intron region in the RasGRP1 gene contains the NBRE (AAAGGTCA) sequence. The black boxes represent the exons. (B) Schematic diagram of the luciferase reporter construct used in the assays. (C) 293 T cells were transfected with either the reporter plasmid alone (250 ng) or a combination of different amounts of the Nurr1 expression vector (50, 100, or 250 ng) using Lipofectamine 2000. After 24 h of transfection, the cells were harvested and lysed, and the luciferase activity was quantified with the dual-luciferase assay system. Renilla activity was used for normalization. This experiment was repeated three times using independently prepared cell lysates. Statistical analysis was performed using Student's t-test (mean ± SD; n = 3; **P < 0.01, ***P < 0.005).