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Nurr1 bound to a specific site in the second intron of the RasGRP1 gene. ChIP assay using an anti-Nurr1 antibody or normal rabbit IgG was performed with BV2 cells, and isolated DNA was subsequently used for PCR with primers flanking the NBRE sequence in the RasGRP1 gene. PCR specificity was confirmed by agarose gel electrophoresis of the PCR products. This experiment was repeated three times (n = 3).
