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. 2020 Jul 1;10:10755. doi: 10.1038/s41598-020-67549-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cas9-mediated deletion of the NBRE sequence in the RasGRP1 intron partially reduced the LPS-induced expression level of proinflammatory cytokine genes and the RasGRP1 gene/protein. (A–C) By Cas9-mediated deletion of the NBRE sequence, Nurr1 partially suppressed the LPS-induced expression level of proinflammatory cytokine genes and RasGRP1 gene. BV2 cells were treated with LPS (1 μg/ml) for 6 h. The expression level of mRNA (A) and protein (B) were analyzed by a semi-quantitative real time PCR and western blot. (C) The expression level of the RasGRP1 protein was quantitatively analyzed by densitometry. These experiments were repeated three times. Statistical analysis was performed using Student's t-test (mean ± SD; n = 3; *P < 0.05, **P < 0.01, ***P < 0.005).