Table 3.

Validation of label-free mass spectrometry data for selected proteins by ELISA and Western blotting.

Cbs/CBS genotype	Mouse					Human
	Gpx3, ng/mL (n)	Pon1, ng/mL (n)	Serpinc1, ng/mL (n)	Mup20a, relative (n)	IgGa, relative (n)	GPX3, ng/mL (n)	SERPINC1, ng/mL (n)
−/−	1.7 ± 0.3 (7)	5.7 ± 1.1 (11)	66.0 ± 5.2 (6)	0.90 ± 0.66 (8)	0.31 ± 0.06 (8)	19.1 ± 4.4 (10)	455 ± 291 (10)
+ / +	1.3 ± 0.2 (9)	9.6 ± 3.6 (10)	55.3 ± 5.1 (5)	0.54 ± 0.73 (8)	1.00 ± 0.14 (8)	16.1 ± 1.5 (10)	723 ± 209 (14)
Fold change	1.32	0.60	1.19	1.67	0.31	1.19	0.63
P valueb	0.0035	0.005	0.019	0.004b	4E−06	< 0.05	< 0.05

Quantified by ELISA.

^aQuantified by Western blotting. ^bCalculated using Log transformed data. ^bTwo-sided t-test.