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. 2020 Jul 1;11(7):496. doi: 10.1038/s41419-020-2526-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — The effects of CHPF over-expression on proliferation, colony formation and apoptosis of A375 cells were examined by Celigo cell counting assay (a), colony formation assay (b) and flow cytometry assay (c), respectively. The effect of CHPF over-expression on cell migration of A375 cells was detected by wound-healing assay (d) and Transwell assay (e). (f–h) The promotion of tumor growth by CHPF over-expression was verified in mice xenograft model. The represented images were selected from at least 3 independent experiments. The data were expressed as mean ± SD (n ≥ 3), *P < 0.05, **P < 0.01, ***P < 0.001.