Figure 5.

Effects of BDNF on the protein levels of Glut2 and Sglt1 (a,b) and on the activity of key enzymes involved in glucose and lipid metabolism (c–i) in ZFL cells. Cells were incubated with culture media alone (control) or containing 1 or 10 nM BDNF during 1 h. In figures (a,b), a representative blot is shown for each protein. Blots shown here were cropped from different parts of a same blot. Full-length blots are presented in SI Fig. S1. Data are shown as mean + SEM (n = 4 for a,b, and n = 8 for c–i). Different letters indicate significant differences (p < 0.05) among groups assessed by one-way ANOVA and SNK test. Acly ATP citrate lyase, BDNF brain-derived neurotrophic factor, Cpt1a carnitine palmitoyltransferase 1a, Fas fatty acid synthase, Gck glucokinase, Glut2 glucose transporter 2, Hoad 3-hydroxyacyl CoA dehydrogenase, Pepck phosphoenolpyruvate carboxykinase, Pk pyruvate kinase, Sglt1 sodium-glucose cotransporter 1.