Table 2.
In vitro effects of the exposure to BDNF on the expression of genes involved in glucose and lipid transport and metabolism and of key transcription factors in ZFL cells.
| 1 h | 6 h | |||||||
|---|---|---|---|---|---|---|---|---|
| Control | BDNF 0.1 nM |
BDNF 1 nM |
BDNF 10 nM |
Control | BDNF 0.1 nM |
BDNF 1 nM |
BDNF 10 nM |
|
| Glucose transport and metabolism | ||||||||
| Transport: | ||||||||
| glut2 | 1.00 ± 0.11a | 1.52 ± 0.59ab | 2.55 ± 0.24b | 5.07 ± 0.84c | 1.00 ± 0.20a | 1.53 ± 0.23ab | 1.75 ± 0.30b | 2.28 ± 0.15b |
| sglt1 | 1.00 ± 0.24a | 2.82 ± 0.03b | 3.92 ± 0.74bc | 5.91 ± 0.39c | 1.00 ± 0.08a | 1.90 ± 0.09b | 1.95 ± 0.35b | 2.42 ± 0.41b |
| Glycolysis: | ||||||||
| gck | 1.00 ± 0.11a | 1.46 ± 0.06b | 2.02 ± 0.25b | 3.48 ± 0.59c | 1.00 ± 0.09a | 1.79 ± 0.10b | 1.85 ± 0.12b | 2.24 ± 0.56b |
| pfkla | 1.00 ± 0.11 | 1.00 ± 0.15 | 1.08 ± 0.08 | 0.71 ± 0.31 | 1.00 ± 0.10 | 1.10 ± 0.19 | 1.38 ± 0.38 | 0.81 ± 0.21 |
| pfklb | 1.00 ± 0.15 | 1.63 ± 0.05 | 1.11 ± 0.13 | 0.98 ± 0.38 | 1.00 ± 0.06 | 1.03 ± 0.23 | 0.99 ± 0.24 | 1.12 ± 0.17 |
| pklr | 1.00 ± 0.25a | 1.52 ± 0.20ab | 2.37 ± 0.35b | 5.79 ± 0.42c | 1.00 ± 0.10a | 2.13 ± 0.06b | 1.75 ± 0.18b | 2.66 ± 0.37b |
| Gluconeogenesis: | ||||||||
| pck1 | 1.00 ± 0.29 | 1.02 ± 0.24 | 1.34 ± 0.29 | 0.61 ± 0.30 | 1.00 ± 0.10 | 1.12 ± 0.05 | 0.95 ± 0.18 | 1.05 ± 0.39 |
| pck2 | 1.00 ± 0.04a | 2.10 ± 0.13b | 2.09 ± 0.16b | 1.99 ± 0.13b | 1.00 ± 0.02a | 2.34 ± 0.19b | 1.90 ± 0.21b | 2.49 ± 0.30b |
| fbp1a | 1.00 ± 0.21a | 1.80 ± 0.47ab | 2.22 ± 0.22b | 3.72 ± 0.40b | 1.00 ± 0.05a | 1.63 ± 0.19b | 1.77 ± 0.04b | 1.98 ± 0.18b |
| fbp1b | 1.00 ± 0.07a | 1.76 ± 0.19b | 1.48 ± 0.28ab | 1.06 ± 0.41ab | 1.00 ± 0.03 | 1.11 ± 0.19 | 0.94 ± 0.08 | 1.19 ± 0.15 |
| g6pcb | 1.00 ± 0.25a | 2.06 ± 0.38b | 1.97 ± 0.21b | 4.05 ± 0.54c | 1.00 ± 0.13a | 4.08 ± 0.70b | 2.14 ± 0.19c | 3.66 ± 0.39b |
| Glycogenolysis: | ||||||||
| glycogen phosphorylase | 1.00 ± 0.19a | 1.70 ± 0.13b | 1.98 ± 0.20b | 2.23 ± 0.21b | 1.00 ± 0.12a | 1.90 ± 0.23b | 2.11 ± 0.28b | 3.11 ± 0.36b |
| Lipid metabolism | ||||||||
| Fatty acid synthesis: | ||||||||
| acly | 1.00 ± 0.09a | 1.10 ± 0.18a | 3.11 ± 0.29b | 3.38 ± 0.25b | 1.00 ± 0.08a | 1.01 ± 0.17ab | 1.42 ± 0.18b | 3.13 ± 0.34c |
| acaca | 1.00 ± 0.11a | 1.07 ± 0.22a | 2.91 ± 0.31b | 2.06 ± 0.15b | 1.00 ± 0.07a | 0.97 ± 0.16a | 1.60 ± 0.27b | 1.86 ± 0.25b |
| fasn | 1.00 ± 0.12a | 1.84 ± 0.31b | 1.48 ± 0.22b | 1.73 ± 0.35b | 1.00 ± 0.24a | 2.18 ± 0.44b | 3.00 ± 0.41b | 2.96 ± 0.41b |
| Β-oxidation: | ||||||||
| cpt1a | 1.00 ± 0.14a | 1.12 ± 0.09a | 1.81 ± 0.23b | 1.99 ± 0.15b | 1.00 ± 0.08a | 0.79 ± 0.13a | 1.84 ± 0.15b | 1.81 ± 0.28b |
| acadm | 1.00 ± 0.15a | 0.61 ± 0.06b | 0.66 ± 0.07b | 0.62 ± 0.08b | 1.00 ± 0.07a | 0.62 ± 0.11b | 1.01 ± 0.09a | 0.94 ± 0.07a |
| echs1 | 1.00 ± 0.08a | 0.86 ± 0.09ab | 0.67 ± 0.04b | 0.73 ± 0.07b | 1.00 ± 0.09a | 0.80 ± 0.07ab | 0.77 ± 0.08b | 0.62 ± 0.04b |
| hadh | 1.00 ± 0.06a | 0.99 ± 0.05a | 0.77 ± 0.04b | 0.78 ± 0.03b | 1.00 ± 0.07a | 0.83 ± 0.09ab | 0.88 ± 0.09ab | 0.65 ± 0.04b |
| Ketogenesis: | ||||||||
| hmgcl | 1.00 ± 0.30a | 1.79 ± 0.37a | 5.46 ± 1.00b | 6.73 ± 1.43b | 1.00 ± 0.11a | 2.63 ± 0.60ab | 3.03 ± 0.47b | 5.42 ± 0.94c |
| acat1 | 1.00 ± 0.09a | 0.92 ± 0.08ab | 1.55 ± 0.17b | 2.77 ± 0.35c | 1.00 ± 0.13a | 1.30 ± 0.28a | 2.74 ± 0.47b | 4.03 ± 0.50c |
| Transcriptional regulators | ||||||||
| ppargc1α | 1.00 ± 0.21a | 1.72 ± 0.50ab | 2.77 ± 0.35bc | 3.71 ± 0.43c | 1.00 ± 0.14a | 2.30 ± 0.32b | 2.35 ± 0.35b | 2.41 ± 0.24b |
| pparα | 1.00 ± 0.21a | 1.51 ± 0.09b | 2.38 ± 0.19c | 2.42 ± 0.23c | 1.00 ± 0.16a | 1.99 ± 0.26b | 1.69 ± 0.14b | 2.23 ± 0.24b |
Cells were incubated with culture media alone (control) or containing 0.1, 1 and 10 nM BDNF during 1 and 6 h. Data obtained by RT-qPCR are shown as mean + SEM of the results obtained in two different experiments (n = 6 in each experiment). Different letters indicate significant differences (p < 0.05) among groups within each time point assessed by one-way ANOVA and SNK test.
acaca, acetyl-CoA carboxylase; acadm, acyl-CoA dehydrogenase medium-chain; acat1, acetyl-CoA acetyltransferase 1; acly, ATP citrate lyase; BDNF, brain-derived neurotrophic factor; cpt1a, carnitine palmitoyltransferase 1a; echs1, enoyl-CoA hydratase short-chain 1; fasn, fatty acid synthase; fbp1a, fructose 1,6-bisphosphatase 1a; fbp1b, fructose 1,6-bisphosphatase 1b; g6pcb, glucose 6-phosphatase b; gck, glucokinase; glut2, glucose transporter 2; hadh, 3-hydroxyacyl CoA dehydrogenase; hmgcl, 3-hydroxy-3-methylglutaryl-CoA lyase; pck1, phosphoenolpyruvate carboxykinase 1; pck2, phosphoenolpyruvate carboxykinase 2; pfkla, phosphofructokinase a; pfklb, phosphofructokinase b; pklr, pyruvate kinase; pparα, peroxisome proliferator-activated receptor alpha; ppargc1α, peroxisome proliferator-activated receptor gamma coactivator 1-alpha; sglt1, sodium-glucose cotransporter 1.