Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jul 1;10:10696. doi: 10.1038/s41598-020-67276-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Selective targeting of cholinergic and GABAergic inputs from basal forebrain to the OB. (A) Left, Coronal section (Bregma 0.74) through BF in a ChAT-Cre mouse injected with a Cre-dependent AAV-ChR2 virus. White lines indicate the outline of HDB and VDB. Right, Magnification of HDB showing labeled ChAT+ neurons. (B) Left, Coronal section (Bregma 0.74) through BF in a GAD2-Cre mouse injected with a Cre-dependent AAV-ChR2 virus. White lines indicate the outline of HDB and VDB. Right, Magnification of HDB/VDB showing somata of GAD + neurons. (C) Immunohistochemical staining of coronal sections of GAD2-Cre:GCaMP6 reporter mice (Bregma 0.74). Double labeled neurons are marked with white arrows. (D) ChR2-EYFP-expressing axon terminals in different layers of the OB 4 weeks after AAV-ChR2-EYFP injection into BF of a ChAT-Cre and GAD-Cre mice. GL: glomerular layer, EPL: external plexiform layer, MCL: mitral cell layer, GCL: granule cell layer. (E) Normalized fluorescence intensity profiles show that axons reaching the external plexiform layer are less prominent in GAD-Cre mice. (F) Schematic of experimental approach. See Materials and Methods for details. (G) Data acquisition. In continuous recordings, action potential waveforms with a signal-to-noise ratio of at least 4 SD above baseline noise were saved to a disk (sample rate 24 kHz) and further isolated using off-line spike sorting. The vertical line indicates onset of odorant stimulation. Isolation of waveforms into three different units by principal components 1 and 2 (bottom, left). Spike waveforms of the isolated units (bottom, right). (H) Inter-spike-interval histograms for two units. One is a single unit (SU) and one a multi unit (MU). Only single units were analyzed further. For units to be classified as presumptive MTCs also a clear sniff-modulation in sniff-triggered spike averages had to be present (inset).