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. 2020 Jul 1;10:10688. doi: 10.1038/s41598-020-67756-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The overexpression vector pChlamy4 CrBKT V5H. Half arrows represent binding positions and 5′-3′ direction of primers used for PCR screening and insertion mapping of transformants. Also shown are the sequences of adaptors and adaptor primers used for insertion mapping. Oligonucleotides 389 and 390 annealed to form asymmetric blunt-ended adaptor. The short strand was extended after first round of PCR.