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. 2020 Jul 1;10:10736. doi: 10.1038/s41598-020-67330-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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T lymphocyte cell populations and ratio in the spleen of chickens infected with E. maxima and housed at thermoneutral (TNi; green) or heat stress (HSi; red) environment, as compared to uninfected chickens housed at thermoneutral (TNc; blue) or heat stress (HSc; yellow) environments. CD4⁺:CD8⁺ ratio at 7 dpi (a), CD4⁺ percentage at 7 dpi (b), CD8⁺ percentage at 7 dpi (c), CD4⁺:CD8⁺ ratio at 14 dpi (d), CD4⁺ percentage at 14 dpi (e), CD8⁺ percentage at 14 dpi (f). Data were analyzed by GLM procedure with multiple comparisons corrected with Tukey’s method at 5% level of significance (p < 0.05). Mean and standard error of the mean (SEM) are depicted. Significant differences between the groups are indicated by different superscript letters.