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. 2020 Jul 1;10:10745. doi: 10.1038/s41598-020-67518-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of GP chondrocyte disarrangement on articular cartilage (AC) in the absence of experimental osteoarthritis (OA). (a) Representative Safranin-O Fast Green stained mice joint sections at × 5 and × 20 magnification in NO-WT and NO-Evc^cKO mice (bars = 250 and 100 µm respectively). (b) Histopathological OARSI Score represented as the sum of tibia and femur scores in NO-WT and NO-Evc^cKO mice. (c) Representative Haematoxilin-Eosin stained tibia growth plate (GP) sections at × 40 magnification in NO-WT and NO-Evc^cKO mice (bars = 50 µm). (d) Percentage of cells in columns and column index (CI) in the GP of NO-WT and NO-Evc^cKO mice. Data are shown as median ± interquartile range (IQR). NO-WT, n = 10–16; NO-Evc^cKO, n = 5.