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. 2020 Jul 1;10:10694. doi: 10.1038/s41598-020-67833-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Inversely regulated miRNA-mRNA pairs during developmental transitions. (a–d) Volcano plots represent the results of differential expression analyses that were performed for mRNAs and miRNAs between successive developmental stages. For each comparison the number (N) of down- (dark blue), upregulated (yellow) and not regulated (grey) mRNAs and miRNAs is indicated. (e) Based on the differential expression results pairs of miRNAs (light blue) and mRNAs (green) identified that are inversely regulated during the transition from tetrads to post-meiotic pollen as well as during the transition from post-meiotic to mature pollen. (f) Assignment of inversely regulated mRNAs to MapMan terms of the second hierarchy level. Here, only Map Man terms with at least 5 assigned mRNAs are shown.