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. 2020 Jul 1;11:3281. doi: 10.1038/s41467-020-17101-y

Fig. 10. Expression of NSs in the brain is responsible for animal death.

Fig. 10

a–e BALB/c mice were inoculated by intracranial injection with 10 and 100 pfu of RVFV and RVFV ΔNSs C13. a, b Brain tissues from animals infected with 100 pfu were stained with anti-N and anti-NSs Abs and visualized with a wide-field scanner. Nuclei appear in blue and the proteins N a and NSs b in red. Experiments were repeated independently thrice with similar results. Scale bars, 10 µm. c Brain from mice inoculated with 10 and 100 pfu of viruses were assessed for infectious particles by plaque-forming assay. Points represent titers of individual animals (n = 5 with the exception of the injection with 10 pfu of RVFV ΔNSs C13, n = 3). Center line, mean. pfu, plaque-forming units. Groups of five 9-week-old BALB/c male mice were inoculated with 100 d and 10 e pfu of viruses and survival of animals was monitored over 10 days. Simple linear regression tests were applied to assess the significance of differences observed between RVFV and RVFV ΔNSs C13. **p = 0.0015 d and 0.0029 e.