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. 2020 Jul 1;10:10733. doi: 10.1038/s41598-020-67645-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Hsp90β’s conformation is more restricted in the human Hsp90 system. (A) Sedimentation velocity AUC analysis of *GRLBDm (grey), *GRLBDm·Hsp90β (red), *GRLBDm·Hsp90β/ATPγS (navy), *GRLBDm·Hsp90β/ATPγS·p23 (light green), *GRLBDm·Hsp90β·Pp5 (yellow), *GRLBDm·Hsp90β/ATPγS·Pp5 (green), *GRLBDm·Hsp90β/ ATPγS·Pp5·p23 (blue) complexes. (B) Sedimentation velocity analysis of *GRLBDm·Hsp90β complexes with Fkbp51 and/or p23 set-up accordingly. (C) Sedimentation velocity analysis of *GRLBDm·Hsp90β complexes with Fkbp52 and/or p23 set-up accordingly. (D) Association kinetics of F-DEX to GRLBDm (grey) in the presence of human Hsp90β (red) and nucleotides as indicated in the plot. (E) Sedimentation velocity analysis of F-DEX association to GRLBDm (grey) in the presence of human Hsp90β (red) and nucleotides as indicated in the plot. (F) Association kinetics of F-DEX tο GRLBDm (grey) in the presence of human Hsp90β/ATP (red) and cofactors as indicated in the plot. G) Sedimentation velocity analysis of F-DEX association to GRLBDm (grey) in the presence of human Hsp90β/ATP (red) and cofactors as indicated in the plot.