Table 2.
Inter-protein crosslinked peptides detected for GRLBDm·HSP-90·FKB-6 complexes.
| Peptide 1 | Peptide 2 | Lys 1 | Lys 2 | MS2 scan | Hit number in scan | Hit % spectral intensity | Independent analysis |
|---|---|---|---|---|---|---|---|
| Peptide HSP-90 | Peptide GR-LBD | ||||||
| APFDLFENKK | ELGKAIAK | 326 | 699 | 24,087 | 38 | 83 | ✓ |
| DSSTMGYMAAKK | QVIAAVKWAK | 601 | 576 | 22,389 | 92 | 74 | ✓ |
| APFDLFENKK | MTYIKELGK | 326 | 695 | 27,231 | 89 | 91 | ✓ |
| SKNSIK | MTYIKELGK | 329 | 695 | 13,803 | 77 | 60 | ✓ |
| VEKVGVSNR | WAKAIPGFR | 555 | 579 | 20,880 | 31 | 55 | ✓ |
| DILEKK | WAKAIPGFR | 551 | 579 | 25,458 | 18 | 44 | ✓ |
| Peptide HSP-90 | Peptide FKB-6 | ||||||
| MKENQTQIYYITGESK | VPATWEMTAEEKLDAAKQAK | 455 | 250 | 23,025 | 30 | 82 | |
| KCMELIDEVAEDKDNFK | MSGEKIDITPKK | 402 | 11 | 20,677 | 23 | 86 | ✓ |
| DNFKK | YKR | 406 | 278 | 25,623 | 18 | 98 |
Shown are the crosslinked products detected by mass spectrometry for GRLBDm·HSP-90· FKB-6 complexes, using the search algorithms, xMASS and pLink. Amino acid positions refer to full length, non-tagged proteins as numbered in the Uniprot database. Scan number refers to the MS2 scan with most hits for the specified peptide. Shown are also the number of hits for this scan, the per cent spectral intensity as well as the agreement of xMASS with the pLink algorithm.