Table 2.
Summary of various production methods of COS and GLcNAc.
| Enzymatic | Chemical | Microbial engineering | Transglycosylation | Chemoenzymatic | |
|---|---|---|---|---|---|
| Reaction conditions | Gentle (Temp.: 37–60°C pH: 3–12) |
Harsh (Conc. acid Temp.:40–200°C for 1–24 h) |
Gentle (Temp: 40–60°C pH:5.5–12) |
Gentle (Temp.: 40- 60°C pH:5–7) |
Mild (Temp.: 100–200°C for 1–6 h) |
| Product yield | Low | High | High | Low | High |
| Specificity | High | Low | High | High | High |
| Toxicity | Non-toxic | High | Non-toxic | Non-toxic | Low to moderately toxic |
| Possibility of industrial implementation | Limited | High but separation of product is costly | High | Limited | High |