Figure 2.

Effect of SPINK5 Disruption on LEKTI Expression

(A) LEKTI immunofluorescence (IF) of human keratinocytes treated with the different RNP pairs. Cells treated with RNP 1+4 and 1+3 show a significant decrease in LEKTI expression compared to their healthy isogenic counterparts (HK Ctrl), resembling NS keratinocytes (HKNS). (B) Western blot analysis confirms the reduction of full-length (145 kDa) and bioactive LEKTI (65 kDa) in ΔSPINK5 cell lysates, consistent with the exon 1 deletion rate and the IF data.