Figure 2.

Vascular phenotypes progress over time. Retina flat mounts of pdx1^−/− mutants and pdx1^+/+ controls, transgenic for fli1a:EGFP, showing representative areas of arteries (A) and capillaries (D) at the ages indicated, from 2 months through 15 months of age. Size bars indicate 50 µm. (B, C) Quantitation of arterial diameter (B) and density (C) in 15 vessels or 15 regions respectively from 4 individual fish per group, from samples as shown in A. m, months, ns, not significant. ***P < 0.001. (E, F) Quantitation of the number of capillary sprouts (E) and branches (F) in 10 capillary regions from 3 individual fish per group, from samples as shown in D. p, pdx1, m, months. **P < 0.01, ***P < 0.001.