FIGURE 1.

Phenotypic analysis of a mutant allele with fertility defects. (A) Plant fertility phenotype of wild type (Ler) and line 184 mutant. Red arrow indicates short silique of the mutant. Bar = 1 cm. (B) Morphology of open flower of wild type (Ler) and line 184 mutant. The mutant has shorter filaments and nearly no pollen grains could be observed on the stigma. (C) Pollen viability (Alexander staining) in wild type and line 184 mutant anthers. Bar = 100 μm. (D) Morphology of microspore tetrad (stained with toluidine blue). Normal meiosis produce tetrad contains four microspores whereas the mutant produce polyads contain more than four microspores. Bar = 10 μm. (E) Chromosome morphology of male meiosis in wild type and line 184. Bar = 5 μm.