Skip to main content
. 2020 Jun 25;11:839. doi: 10.3389/fpls.2020.00839

FIGURE 5.

FIGURE 5

Number of chiasmata in wild type and atm mutants. (A) Bivalents in metaphase I meiocytes in wild type and atm mutants. The number of chiasmata of each chromosome was marked aside. (B) Number of chiasmata per cell in wild type (10.85 ± 1.18, n = 20) and atm mutants (atm-2: 12 ± 1.02, n = 25, P = 2.72E-3; atm-5: 12.01 ± 1.37, n = 21, P = 7.76E-3, Wilcoxon Rank Sum test). For each allele, metaphase I cells used for chiasmata counting are from about 30 plants. The atm-2 and atm-5 are two independent atm mutant alleles. (C) Immunolocalization of HEI10 (green dots) on pachytene (atm-2: 11.3 ± 1.82, n = 113, P = 7.59E-13; atm-5: 11.05 ± 1.47, n = 65, P = 3.25E-09) and diakinesis (atm-2: 11.25 ± 1.49, n = 110, P = 2.18E-04; atm-5: 10.94 ± 1.41, n = 48, P = 7.17E-03) stage chromosomes (magenta) in wild type and atm mutants. Bar = 5 μm. (D) Number of HEI10 foci in wild type and atm mutants. Cells counted in each allele are from at least 30 plants. The atm-2 and atm-5 are two independent atm mutant alleles.