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. 2020 Jun 25;11:1383. doi: 10.3389/fmicb.2020.01383

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Copyright © 2020 Binder, Shadkchan, Osherov and Krappmann.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Generation of a conditional promoter replacement (CPR) strain to modulate trxR expression validates essentiality of the thioredoxin reductase-encoding gene for A. fumigatus. (A) Schematic outline of the CPR procedure employing the tetOFF:trxR module of pSK655. (B) Steady state trxR transcript levels in the wild-type strain AfS35 and its tetOFF:trxR derivative AfS227 illustrate a significant down-regulation of trxR transcription for the latter in the presence of doxycycline (+Dox). (C) Growth of AfS227 on minimal (left panel) and rich Sabouraud (right panel) culture medium is strongly inhibited by doxycycline, thereby validating essentiality of the trxR gene.