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. 2020 Jun 25;11:581. doi: 10.3389/fphys.2020.00581

FIGURE 2.

FIGURE 2

Quantification of RAGE and HMGB1 expression in human fetal membranes at term in the ZIM and ZAM. RAGE and HMGB1 expression were quantified by RT-qPCR (N = 14) (A) and Western blot (respectively, N = 11 and N = 8) (B) on the amnion and choriodecidua (Cd) at term in the ZIM and ZAM. To highlight an area (ZAM vs. ZIM) or tissue (Amnion vs. Choriodecidua) effect, ZAM is reported on ZIM (ZAM/ZIM in left panel) in both amnion and choriodecidua, and amnion is reported on choriodecidua (amnion/choriodecidua in right panel) in both ZIM and ZAM. Statistical fold change analysis was performed by a Wilcoxon signed-rank test comparing to one. * means p < 0.05, ** means p < 0.01, *** means p < 0.001. Results are presented in Tukey boxes, and means are indicated by “+.” Representative Western blot membranes indicate the band that was quantified proteins (RAGE: 50 kDa and HMGB1 at 25 kDa).