TABLE 3.
Differentially expressed genes associated with the lysosome pathway.
| Gene ID | Log2 ratio (T/C) | Regulation (T/C) | P-value | Protein | Phenotype after RNAi of DEGs in T. castaneuma |
| LOC658343 | 2.92 | Up | 1.06E-24 | CP 1 | / |
| LOC660669 | 2.62 | Up | 8.30E-75 | Cat L precursor | / |
| LOC659367 | 2.08 | Up | 1.84E-77 | Cat L precursor | / |
| LOC664569 | 2.04 | Up | 0 | Beta-hexosaminidase subunit alpha | / |
| LOC663215 | 1.96 | Up | 3.22E-17 | MFS15 | / |
| LOC657117 | 1.81 | Up | 1.26E-06 | Cat B1 like | / |
| LOC107398158 | 1.79 | Up | 7.41E-05 | Cat L1 like | / |
| LOC100141821 | 1.48 | Up | 4.30E-12 | Snmp1 | / |
| LOC659039 | 1.41 | Up | 3.36E-28 | Lipase member K | / |
| LOC103312286 | 1.30 | Up | 5.53E-295 | Lipopolysaccharide | / |
| LOC664564 | 1.28 | Up | 3.89E-07 | Glucosylceramidase like | / |
| LOC660551 | 1.26 | Up | 1.04E-307 | Cat L | / |
| LOC103313498 | 1.10 | Up | 0 | Crammer | / |
| LOC103314303 | 1.03 | Up | 1.29E-19 | CD63 antigen like | / |
| LOC661588 | 1.03 | Up | 4.25E-67 | Arylsulfatase B | / |
| LOC656698 | −1.11 | Down | 2.40E-18 | Lysosomal alpha-mannosidase like | / |
| LOC657825 | −1.12 | Down | 1.27E-15 | BG | / |
| LOC660368 | −1.16 | Down | 3.69E-175 | Cat L precursor | 100%b, 40% vitellogenic egg chamber not presentc |
| LOC662197 | −1.28 | Down | 9.74E-90 | Alpha-N-acetylgalactosaminidase | / |
| LOC663117 | −1.30 | Down | 7.73E-28 | Cat B1 | / |
| LOC659525 | −1.32 | Down | 5.41E-84 | Arylsulfatase B like | / |
| LOC655752 | −1.36 | Down | 9.57E-12 | Serine protease P38 | / |
| LOC103312725 | −1.63 | Down | 3.19E-11 | ML | / |
| LOC661631 | −1.75 | Down | 8.99E-81 | Lipase 1 | / |
| LOC659587 | −1.78 | Down | 0 | Arylsulfatase B like | / |
| LOC659121 | −1.82 | Down | 2.14E-132 | Lipase 1 like | 100% previtellogenic egg chamber orientation irregularc |
| LOC661718 | −2.14 | Down | 6.36E-24 | Lipase 3 like | / |
| LOC661966 | −2.97 | Down | 2.16E-296 | Lipase 4 | 20%b |
C, control; T, 5% A. vulgaris treatment; CP, cysteine proteinase; Cat, cathepsin; Snmp1, sensory neuron membrane protein 1; MFS15, major facilitator superfamily transporter 15; BG, beta-glucuronidase; ML, MD-2-related lipid-recognition; ametamorphosis and survival after RNAi were analyzed (http://ibeetle-base.uni-goettingen.de); bLethalities 11 days after larval injection (includes death as larva, prepupa, pupa); c13 days after female pupal injection.